Electrochemical Biosensing Platforms on Basis of Reduced Graphene Oxide and Its Composites with Au Nanodots
Reduced graphene oxide (rGO) and Au nanodots (AuNDs, ~1.5 nm) modified rGO, referred to as AuNDs-rGO, synthesized by a simple photochemical reduction method, are used for electrochemical biosensors. Our experiment results showed that the glucose oxidase (GOX)/rGO/Nafion/glassy carbon electrode (GCE) exhibits linear range from 0.03 to 6.28 mM and detection limit of 0.03 mM at a signal-to-noise (S/N) ratio of 3 in the amperometric sensing of glucose oxidase, while the GOX/AuNDs-rGO/Nafion/GCE shows wider linear range from 0.02 to 8.82 mM and lower detection limit of 0.02 mM. This difference may arise from the high catalytic activity of the AuNDs. Our studies demonstrate that the composites of rGO/Nafion and AuNDs-rGO/Nafion might offer a new class of electrode materials, and provide a promising platform for biosensing applications.