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Issue 21, 2020
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Native detection of protein O-GlcNAcylation by gel electrophoresis

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Abstract

O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of O-GlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.

Graphical abstract: Native detection of protein O-GlcNAcylation by gel electrophoresis

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Article information


Submitted
12 Dec 2019
Accepted
30 Apr 2020
First published
02 Sep 2020

This article is Open Access

Analyst, 2020,145, 6826-6830
Article type
Communication

Native detection of protein O-GlcNAcylation by gel electrophoresis

C. Fu and D. M. F. van Aalten, Analyst, 2020, 145, 6826
DOI: 10.1039/C9AN02506E

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