Jump to main content
Jump to site search


A near-infrared phosphorescent iridium(iii) complex for fast and time-resolved detection of cysteine and homocysteine

Author affiliations

Abstract

Thiol-containing amino acids, cysteine (Cys) and homocysteine (Hcy), play crucial roles in the biosystem; their abnormal contents in the cells are linked to many diseases. Herein, we designed and synthesized a novel near-infrared (NIR) phosphorescent iridium(III) complex-based probe (FNO1) that can detect Cys and Hcy in real-time in the biosystem. Due to the advantages of the iridium complex, the FNO1 probe had excellent chemical stability and photostability, high luminescence efficiency, and long luminescence lifetime. In addition, the probe showed a fast response, high sensitivity, and low cytotoxicity. As verified by high resolution mass spectra (HR-MS) and density functional theory (DFT) calculations, the detection was achieved through the addition of the α,β-unsaturated ketone group in FNO1 by the nucleophilic thiol group in Cys and Hcy. Through time-resolved emission spectroscopy (TRES) and in the presence of a strongly fluorescent dye rhodamine B, the FNO1 probe could detect Cys and Hcy due to its long luminescence lifetime (260/197 ns). Finally, owing to its NIR-emitting properties, the FNO1 probe was successfully applied in the imaging of Cys and Hcy in living cells, zebrafish, and mice.

Graphical abstract: A near-infrared phosphorescent iridium(iii) complex for fast and time-resolved detection of cysteine and homocysteine

Back to tab navigation

Supplementary files

Article information


Submitted
07 Dec 2019
Accepted
28 Jan 2020
First published
31 Jan 2020

Analyst, 2020, Advance Article
Article type
Paper

A near-infrared phosphorescent iridium(III) complex for fast and time-resolved detection of cysteine and homocysteine

Y. Li, Y. Wu, J. Wu, W. Lun, H. Zeng and X. Fan, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02469G

Social activity

Search articles by author

Spotlight

Advertisements