A ratiometric fluorescent probe for reversible monitoring of endogenous SO2/formaldehyde in cytoplasm and nucleoli regions and its applications in living mice
Excessive production sulfur dioxide (SO2) and formaldehyde (FA) by organisms are closely associated with several diseases including neurological disorders, respiratory disease and genotoxicity. RNA plays a central role in the expression of gene. However, it is difficult for fluorescent probes to explore the intersecting correlation of SO2 and FA near the nucleolus regions due to the protective obstacle of the cell nucleus membrane. Herein, a unique fluorescent probe (DP) for reversible detecting the interaction of SO2 and FA in nucleolus regions was constructed. In light of the outstanding attributes of DP including large fluorescence ratio variation, ultrafast detection of SO2 and excellent reversibility, it was successfully employed to track the variation of endogenous SO2 and FA in cytoplasm and nucleolus regions of live cell. In addition, the probe DP can be applied to imaging of endogenous SO2 and recovered by FA in live mice.