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Imaging stressed organelles via sugar-conjugated color-switchable pH sensors

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Abstract

Stressed organelles are often challenging to image with canonical organelle probes owing to their propensity to dissipate from stressed organelles. We herein report the imaging of stressed lysosomes with color-switchable glyco-probes that contain an entity of mannose-6-carboxylate or sialic acid for targeting to and long-term retention in stressed lysosomes, and a diad of fluorescein/rhodamine-X-lactam exhibiting dramatic red-to-green fluorescence shift upon pH elevation. Relative to acidotropic dyes prone to dissipate from pH-elevated lysosomes, both glyco-probes are stably trapped in lysosomes without resorting to lysosomal acidity. Importantly, these probes are red emissive in acidic lysosomes (pH 4.5–5.8), but switched to green fluorescence in lysosomes of pH 6.0–7.4, allowing dual color discrimination of lysosomal pH alterations in cell starvation. These results support the use of sugar-armed sensors to investigate stressed lysosomes in biology and disease.

Graphical abstract: Imaging stressed organelles via sugar-conjugated color-switchable pH sensors

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Article information


Submitted
04 Dec 2019
Accepted
03 Jan 2020
First published
04 Jan 2020

Analyst, 2020, Advance Article
Article type
Paper

Imaging stressed organelles via sugar-conjugated color-switchable pH sensors

E. Zhang, S. Wang, X. Su and S. Han, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02441G

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