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A high selectivity fluorescent probe for hypoxia imaging in cells and tumor-bearing mice model

Abstract

Nitroreductase (NTR) with high expression level in tumor has been considered as a biomarker of highly aggressive hypoxia tumor. Thus, it is important to develop powerful tools for tumor hypoxia detection. Here, we developed a two-photon fluorescent probe hTP-NNO2 for NTR detection. The probe with one-step synthesis exhibited high yield. hTP-NNO2 showed high selectivity and sensitivity for NTR and the detection limit was as low as 43 ng/mL. hTP-NNO2 also displayed low cytotoxicity and high stability, indicating that hTP-NNO2 was suitable for NTR detection in real-time and in situ under physiological conditions. hTP-NNO2 was applied for NTR imaging in hypoxia cells and the fluorescence intensity of hTP-NNO2 increased with decreasing oxygen concentration. Benefiting from the advantages of two-photon fluorescent probes, we performed NTR detection in deep brain tissue and the imaging depth up to 100 μm. hTP-NNO2 was further successfully applied for NTR detection in zebrafish and tumors. The results indicated that we developed a promising fluorescence imaging tool for NTR detection in vitro and in vivo.

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Supplementary files

Article information


Submitted
03 Dec 2019
Accepted
06 Jan 2020
First published
07 Jan 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

A high selectivity fluorescent probe for hypoxia imaging in cells and tumor-bearing mice model

Y. Wang, X. Han, X. Zhang, L. Zhang and L. Chen, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/C9AN02436K

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