A high selectivity fluorescent probe for hypoxia imaging in cells and tumor-bearing mice model
Nitroreductase (NTR) with high expression level in tumor has been considered as a biomarker of highly aggressive hypoxia tumor. Thus, it is important to develop powerful tools for tumor hypoxia detection. Here, we developed a two-photon fluorescent probe hTP-NNO2 for NTR detection. The probe with one-step synthesis exhibited high yield. hTP-NNO2 showed high selectivity and sensitivity for NTR and the detection limit was as low as 43 ng/mL. hTP-NNO2 also displayed low cytotoxicity and high stability, indicating that hTP-NNO2 was suitable for NTR detection in real-time and in situ under physiological conditions. hTP-NNO2 was applied for NTR imaging in hypoxia cells and the fluorescence intensity of hTP-NNO2 increased with decreasing oxygen concentration. Benefiting from the advantages of two-photon fluorescent probes, we performed NTR detection in deep brain tissue and the imaging depth up to 100 μm. hTP-NNO2 was further successfully applied for NTR detection in zebrafish and tumors. The results indicated that we developed a promising fluorescence imaging tool for NTR detection in vitro and in vivo.