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A highly sensitive immunoassay of pesticide and veterinary drug residues in food by tandem conjugation of bi-functional mesoporous silica nanospheres

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Abstract

A novel type of enzyme–antibody conjugation using mesoporous silicon nanospheres (MSN) was developed, which amplified the labeling signal and highly increased the sensitivity of enzyme-linked immunosorbent assay (ELISA) for the determination of pesticide and veterinary drug residues in food. First, conjugates were prepared through layer-by-layer immobilization of an enzyme and an antibody on an MSN scaffold. Then the MSN scaffold was employed for labeling and signal amplification to develop a sensitive colorimetric immunoassay through the catalytic oxidation reaction of 5,50-tetramethylbenzidine (TMB). When this MSN-based ELISA was applied to detect chloramphenicol, avermectin, tetracycline and streptomycin in food samples, it provided linear ranges of 0.025 ng ml−1–25 ng ml−1, 0.05 ng ml−1–10 ng ml−1, 0.025 ng ml−1–10 ng ml−1 and 0.05 ng ml−1–25 ng ml−1, respectively, with low detection limits down to 0.011 ng mL−1, 0.134 ng mL−1, 0.015 ng ml−1 and 0.106 ng ml−1, respectively. For avermectin, it provided a 16.7-fold decrease of the limit of detection in contrast to that of standard ELISA without the loss of method specificity and accuracy. This novel immunoassay was hypersensitive, simple and easy-to-use, which made it high potential in applying for the accurate analysis of harmful substances in food.

Graphical abstract: A highly sensitive immunoassay of pesticide and veterinary drug residues in food by tandem conjugation of bi-functional mesoporous silica nanospheres

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Article information


Submitted
03 Dec 2019
Accepted
16 Jan 2020
First published
18 Jan 2020

Analyst, 2020, Advance Article
Article type
Paper

A highly sensitive immunoassay of pesticide and veterinary drug residues in food by tandem conjugation of bi-functional mesoporous silica nanospheres

H. Ji, C. Xia, J. Xu, X. Wu, L. Qiao and C. Zhang, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02430A

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