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Copper-based metal-organic framework/graphene nanocomposite for sensitive and stable electrochemical detection of DNA bases

Abstract

A simple and easy-operation electrode modification strategy was proposed using Cu-MOF/GO nanohybid for physiologists and pathologists to the feasible and reliable simultaneous electrochemical detections of DNA bases, guanine and adenine. The nanohybid was prepared by simple ultrasonic method and was employed for fabrication of sensing interface. SEM, TEM, XRD, FT-IR and electrochemical characterizations were used to characterize general morphology and the structure of the nonohybrid. The proposed Cu-MOF/ERGO/GCE shows ultra-stable and high-sensitivity performance in simultaneous electrochemical detection of guanine and adenine. The recorded DPV curves reveal a linear increase in faradic signals with increases in concentrations of guanine and adenine in the range of 0.02−10 µM and 20−100 µM for guanine, and 0.005−20 µM and 40−200 µM for adenine.The relative standard deviation of guanine and adenine for 50 consecutive detection is 1.37% and 1.92%, respectively. It was proved that the proposed Cu-MOF/ERGO/GCE can be performed to the detection of guanine and adenine in real samples, Herring sperm DNA, and satisfactory results were obtained. This strategy does not require complicated modification procedures, professional modification techniques, or sophisticated instruments, but it can provide a highly sensitive and stable detection method, which is expected to expand and deepen the application of electrochemical detection in life science research.

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Supplementary files

Article information


Submitted
28 Nov 2019
Accepted
07 Jan 2020
First published
08 Jan 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

Copper-based metal-organic framework/graphene nanocomposite for sensitive and stable electrochemical detection of DNA bases

X. Wang, J. Zhang, Y. Wei, T. Xing, T. Cao, S. Wu and F. Zhu, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/C9AN02398D

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