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A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

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Abstract

We developed a microfluidic gradient device to utilize as a drug screening system with human induced pluripotent stem cell (hiPSC)-derived motoneurons. The microfluidic channel was asymmetrically designed to generate the concentration gradients and a micropillar array was used to trap and culture the motoneuron spheroids containing motoneurons for 9 days. We optimized the concentration gradients in the microfluidic device using a computational fluid dynamics (CFD) model. We also observed that the motoneuron spheroid-derived neurite network was generated in response to the concentration gradients of riluzole in the microfluidic device. Therefore, this microfluidic gradient device could be useful for screening of various drugs for neurological disease applications.

Graphical abstract: A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

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Article information


Submitted
26 Nov 2019
Accepted
16 Feb 2020
First published
17 Feb 2020

Analyst, 2020, Advance Article
Article type
Paper

A microfluidic gradient device for drug screening with human iPSC-derived motoneurons

S. J. Mo, J. Lee, H. G. Kye, J. M. Lee, E. Kim, D. Geum, W. Sun and B. G. Chung, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02384D

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