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An enzyme-free and label-free visual sensing strategy for the detection of thrombin using a plasmonic nanoplatform

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Abstract

An enzyme-free and label-free visual sensing strategy was developed for sensitively detecting thrombin using a plasmonic nanoplatform. Both the thrombin-triggered catalytic hairpin assembly (CHA) amplification reaction and G-quadruplex/hemin DNAzyme-controlled plasmonic signal readout were engineered on an electrospun nanofibrous membrane. Owing to its large specific surface area and porous structure, the nanofibrous membrane enhanced the loading capacity of B-H2 and the interface interaction efficiency. This plasmonic nanoplatform was used to perform the sensitive and naked-eye detection of thrombin as low as 1.0 pM in human serum samples. This visual strategy can discriminate thrombin from other co-existing proteins very well. Moreover, the visual sensing platform exhibited excellent reusability and long-term stability. The proposed enzyme-free and label-free plasmonic nanoplatform is low-cost, easy to operate and highly sensitive, and has potential applications in the point-of-care detection of protein biomarkers.

Graphical abstract: An enzyme-free and label-free visual sensing strategy for the detection of thrombin using a plasmonic nanoplatform

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Article information


Submitted
21 Nov 2019
Accepted
12 Jan 2020
First published
24 Jan 2020

Analyst, 2020, Advance Article
Article type
Paper

An enzyme-free and label-free visual sensing strategy for the detection of thrombin using a plasmonic nanoplatform

C. Yin, D. Jiang, D. Xiao and C. Zhou, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02340B

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