Jump to main content
Jump to site search


Bioaccumulation investigation of bisphenol A in HepG2 cells and zebrafishes enabled by cobalt magnetic polystyrene microsphere derived carbon based magnetic solid-phase extraction

Author affiliations

Abstract

A magnetic solid-phase extraction (MSPE) technique coupled with high performance liquid chromatography (HPLC) was developed and used for bioaccumulation investigation of bisphenol A (BPA) in HepG2 cells and zebrafishes. Cobalt magnetic polystyrene microsphere derived carbon (C–Co@PST) as an adsorbent was prepared by in situ polymerization reaction and further annealing treatment. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy and X-ray diffraction were employed to confirm successful synthesis of C–Co@PST. A series of extraction parameters including the amount of the sorbent, the type of elute, extraction time and elution time were investigated to achieve high extraction efficiency. C–Co@PST based MSPE combined with HPLC was successfully established for bioaccumulation research of BPA in living creatures. It was found that the bioconcentration values of BPA in HepG2 cells underwent an increase, then a decrease, and finally reached an equilibrium level of 11.60 μg kg−1 at 8 h. The concentration of BPA in zebrafishes increased ranging from 6.05 μg kg−1 to 31.84 μg kg−1 over a culture time from 1 h to 12 h. Furthermore, linear and exponential models were employed to analyse the bioconcentration variation of BPA in organisms over the exposure time. Mathematical models have been developed to predict the transfer characteristics of BPA.

Graphical abstract: Bioaccumulation investigation of bisphenol A in HepG2 cells and zebrafishes enabled by cobalt magnetic polystyrene microsphere derived carbon based magnetic solid-phase extraction

Back to tab navigation

Supplementary files

Article information


Submitted
19 Nov 2019
Accepted
11 Dec 2019
First published
12 Dec 2019

Analyst, 2020, Advance Article
Article type
Paper

Bioaccumulation investigation of bisphenol A in HepG2 cells and zebrafishes enabled by cobalt magnetic polystyrene microsphere derived carbon based magnetic solid-phase extraction

P. Niu, X. Lu, B. Liu, Y. Li, X. Liang, S. Wang and Y. Guo, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02324K

Social activity

Search articles by author

Spotlight

Advertisements