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A fluorescence assay for microRNA let-7a by a double-stranded DNA modified gold nanoparticle nanoprobe combined with graphene oxide

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Abstract

Graphene oxide (GO) is capable of quenching fluorescence in bound fuel single stranded DNA (ssDNA) labeled with dye. In the presence of target microRNA let-7a, two cascaded toehold-mediated strand displacement reactions (TSDRs) were triggered, thereby desorbing substantial π–π stacking adsorbed fuel DNA from GO through recycling amplification, simultaneously accompanied with the restoration of dye fluorescence. By coupling dsDNA–AuNPs with the GO nanosheet, the proposed strategy achieved a non-enzymatic sensitive assay with a low detection limit of 3.9 pM. Hence, the proposed approach has great potential for applications in early cancer diagnosis and clinical analysis.

Graphical abstract: A fluorescence assay for microRNA let-7a by a double-stranded DNA modified gold nanoparticle nanoprobe combined with graphene oxide

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Article information


Submitted
12 Nov 2019
Accepted
10 Dec 2019
First published
10 Dec 2019

Analyst, 2020, Advance Article
Article type
Communication

A fluorescence assay for microRNA let-7a by a double-stranded DNA modified gold nanoparticle nanoprobe combined with graphene oxide

Y. Gao, J. Tian, X. Zhang, B. Qiao, Y. Cao, X. Wang and Q. Wu, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02274K

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