Jump to main content
Jump to site search


Paper-based colorimetric assay system for sensitive cysteine detection using fluorescent probe

Abstract

The fluorescent probe for the colorimetric detection of cysteine (Cys) was synthesized by combining resorufin with 7-nitrobenzofurazan. The resultant probe was characterized by FT-IR, fluorescence, mass spectrometry and NMR. After reacting with Cys, the resorufin is released and the color changes from light yellow to red on the paper-base analytical devices. The results were recorded using a common cell phone and subsequently analyzed using Photoshop software. The color intensity of RGB channel increased linearly in the 0.04 to 70.04 M Cys concentration range when probe concentration was 2.6 mM (R=0.9965), and the limit of detection was 16 nM. The effects of detection conditions have been investigated and were discussed. The interference deviations of 13 substances to Cys were in the range of −2% to +2%. The Cys concentration was determined as (270.8  19.3) M in human plasma, and the recovery was from 99.7% to 100.4%. The work demonstrates the potential of the method to detect Cys in real samples with low cost and high sensitivity.

Back to tab navigation

Article information


Submitted
12 Nov 2019
Accepted
14 Jan 2020
First published
15 Jan 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

Paper-based colorimetric assay system for sensitive cysteine detection using fluorescent probe

J. Zhang, Y. Miao, Z. Cheng, L. Liang, X. Ma and C. Liu, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/C9AN02271F

Social activity

Search articles by author

Spotlight

Advertisements