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Issue 7, 2020
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Metabolic mapping with plasmonic nanoparticle assemblies

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Abstract

A rapid and simple methodology for the biomolecular analysis of single cells and microenvironments via a stick-and-peel plasmonic sensing platform is reported. Substrate-bound assemblies of plasmonic gold nanoparticles linked by reconfigurable oligonucleotides undergo disassembly upon target binding. Changes in the light scattering intensity of thousands of discrete nanoparticle assemblies are extrapolated concomitantly to yield the mapping of local target concentrations. The methodology is completely free of labelling, purification and separation steps. We quantified the intracellular ATP levels for two ovarian cancer cell lines to elucidate the differences and cellular distribution, and demonstrated the potential of the stick-and-peel platform for mapping the microenvironment of a 2D heterogeneous surface. The portable and economical analytical platform may broaden the affordability and applicability of single-cell based analyses and enable new opportunities in clinical care such as on-site molecular pathology.

Graphical abstract: Metabolic mapping with plasmonic nanoparticle assemblies

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Supplementary files

Article information


Submitted
11 Nov 2019
Accepted
23 Jan 2020
First published
17 Mar 2020

This article is Open Access

Analyst, 2020,145, 2586-2594
Article type
Paper

Metabolic mapping with plasmonic nanoparticle assemblies

N. H. Le, G. Ye, C. Peng and J. I. L. Chen, Analyst, 2020, 145, 2586 DOI: 10.1039/C9AN02262G

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