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Issue 2, 2020
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A simple microfluidic device for live-imaging of the vertical section of epithelial cells

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Abstract

We investigated the capability of simple microfluidic devices with trenches having vertical sidewalls for live-cell fluorescence imaging of adherent cells. An epithelial cell line that forms a two-dimensional (2D) sheet was cultured to adhere to the vertical sidewall so that its vertical section can be imaged directly using ordinal inverted-type laser-scanning microscopy. The material and the structure of the device were characterized. We show that the detailed distribution of intracellular organelles, such as microtubules and mitochondria, and of intercellular apparatus, such as claudin and zonula occludens, can be imaged with high spatio-temporal resolution with a single scan.

Graphical abstract: A simple microfluidic device for live-imaging of the vertical section of epithelial cells

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Article information


Submitted
28 Oct 2019
Accepted
09 Nov 2019
First published
11 Nov 2019

Analyst, 2020,145, 667-674
Article type
Paper

A simple microfluidic device for live-imaging of the vertical section of epithelial cells

S. Araki, M. Nakano, M. Tsugane, F. Sunaga, M. Hattori, M. Nakano, T. Nagai and H. Suzuki, Analyst, 2020, 145, 667
DOI: 10.1039/C9AN02165E

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