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A simple microfluidic device for live-imaging of the vertical section of epithelial cells

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Abstract

We investigated the capability of simple microfluidic devices with trenches having vertical sidewalls for live-cell fluorescence imaging of adherent cells. An epithelial cell line that forms a two-dimensional (2D) sheet was cultured to adhere to the vertical sidewall so that its vertical section can be imaged directly using ordinal inverted-type laser-scanning microscopy. The material and the structure of the device were characterized. We show that the detailed distribution of intracellular organelles, such as microtubules and mitochondria, and of intercellular apparatus, such as claudin and zonula occludens, can be imaged with high spatio-temporal resolution with a single scan.

Graphical abstract: A simple microfluidic device for live-imaging of the vertical section of epithelial cells

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Publication details

The article was received on 28 Oct 2019, accepted on 09 Nov 2019 and first published on 11 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN02165E
Analyst, 2020, Advance Article

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    A simple microfluidic device for live-imaging of the vertical section of epithelial cells

    S. Araki, M. Nakano, M. Tsugane, F. Sunaga, M. Hattori, M. Nakano, T. Nagai and H. Suzuki, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02165E

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