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Bioluminescence imaging of carbon monoxide in living cells based on a selective deiodination reaction

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Abstract

D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP. It is used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications. Herein, the iodination of D-luciferin was undertaken and explored as a bioluminescence probe without the need for light excitation to sensitively trace and image carbon monoxide (CO) in liver cancer cells. The bioluminescent probe (7′-iodo-luciferin) exhibited excellent selectivity for CO detection in vitro. This new probe could image exogenous and endogenous CO in the luciferase-transfected cancer cells. This new probe might be used for evaluating the roles of CO in various biological processes.

Graphical abstract: Bioluminescence imaging of carbon monoxide in living cells based on a selective deiodination reaction

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Publication details

The article was received on 22 Oct 2019, accepted on 04 Nov 2019 and first published on 07 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN02107H
Analyst, 2020, Advance Article

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    Bioluminescence imaging of carbon monoxide in living cells based on a selective deiodination reaction

    A. Wang, X. Li, Y. Ju, D. Chen and J. Lu, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02107H

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