Issue 3, 2020

A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

Abstract

Rapid detection of physiological changes of neuropeptides is of great importance as they are involved in a wide range of physiological processes and behaviors. Abnormalities in their expression level are correlated with various neurological diseases. However, current methods such as radioimmunoassay, enzyme-linked immunosorbent assays and liquid chromatography tandem mass spectrometry relied on cumbersome operation steps and could not rapidly provide the information of their concentration fluctuations. Thus motivated, we developed a target-driven DNA-based molecular machine that could be triggered only in the presence of a specific target neuropeptide. Using arginine-vasopressin (AVP) as a model neuropeptide, we integrated the DNA-based molecular machine with fluorescence signal transduction and amplification technology. The assay was rapid and homogeneous, which offered a linear range of 75–700 pM and a limit-of-detection as low as 75 pM. It holds great potential for further applications in real-time monitoring of the variations of the AVP level in biological samples.

Graphical abstract: A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

Supplementary files

Article information

Article type
Paper
Submitted
15 Oct 2019
Accepted
19 Nov 2019
First published
21 Nov 2019

Analyst, 2020,145, 880-886

A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin

H. Tan, L. Chen, X. Li, M. Li and M. Zhao, Analyst, 2020, 145, 880 DOI: 10.1039/C9AN02060H

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