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A near infrared fluorescent probe based on ICT for monitoring mitophagy in living cells

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Abstract

Mitophagy, the process in which cells degrade dysfunctional organelles and recycle their nutrient substances by lysosomes, plays a vital role in cell metabolism and physiology. Herein, we present a highly targeting and near-infrared (NIR) mitochondrion fluorescent probe, which can monitor the process of autophagy. The response mechanism of the probe is based on intramolecular charge transfer (ICT) for the detection of autophagy and real-time imaging of living cells. We designed a primary amine as a pH sensitizing group, and due to the ICT process, the probe exhibits green fluorescence, and when it is protonated the ICT process is broken, and the NIR fluorescence will be restored. Simultaneously, the green fluorescence of the probe disappears. This probe exhibits excellent selectivity, high sensitivity and clean responsiveness, which indicate that it can be applied for high-targeting and high-sensitive imaging of the process of autophagy in living systems.

Graphical abstract: A near infrared fluorescent probe based on ICT for monitoring mitophagy in living cells

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Supplementary files

Article information


Submitted
13 Oct 2019
Accepted
01 Dec 2019
First published
03 Dec 2019

Analyst, 2020, Advance Article
Article type
Paper

A near infrared fluorescent probe based on ICT for monitoring mitophagy in living cells

W. Tang, Y. Dai, B. Gu, M. Liu, Z. Yi, Z. Li, Z. Zhang, H. He and R. Zeng, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN02053E

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