Engineering DNAzymes Cascade for Signal Transduction and Amplification
Inspired by natural enzyme cascades reaction, a multiple DNAzymes cascade platform is engineered to imitate intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cut the report substrate S2 to output signals. Thus, the signal is passed from upstream DNAzyme to downstream DNAzyme through a well-designed intermediary, accomplishing the mission of signal transduction and signal amplification. According to the experimental result, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that of single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuit and precision medicine.