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Engineering DNAzymes Cascade for Signal Transduction and Amplification

Abstract

Inspired by natural enzyme cascades reaction, a multiple DNAzymes cascade platform is engineered to imitate intracellular process of signal transduction and signal amplification. In this design, when particular stimuli appear, activated upstream DNAzyme will cleave a well-designed intermediary S1, releasing a downstream DNAzyme that can cut the report substrate S2 to output signals. Thus, the signal is passed from upstream DNAzyme to downstream DNAzyme through a well-designed intermediary, accomplishing the mission of signal transduction and signal amplification. According to the experimental result, the DNAzyme cascades are capable of improving sensitivity for bioassays compared with that of single DNAzyme-based biocatalysis, which holds promise for potential applications, such as biomolecular computing, logic circuit and precision medicine.

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Article information


Submitted
08 Oct 2019
Accepted
07 Jan 2020
First published
10 Jan 2020

Analyst, 2020, Accepted Manuscript
Article type
Paper

Engineering DNAzymes Cascade for Signal Transduction and Amplification

J. Li, K. Quan, Y. Yang, X. Yang, X. Meng, J. Huang and K. Wang, Analyst, 2020, Accepted Manuscript , DOI: 10.1039/C9AN02003A

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