Rapid and Specific Duplex Detection of Methicillin-resistant Staphylococcus aureus Genes by Surface-enhanced Raman Spectroscopy
Methicillin-resistant Staphylococcus aureus (MRSA) is considered one of the important hospital-acquired pathogens. MRSA is also commonly associated with hospital-acquired infections and mortality. Providing quantitative and precise detection of MRSA is essential for rapid diagnosis and subsequent effective disease management strategies. We herein developed a highly specific method for rapid MRSA detection that combines surface-enhanced Raman spectroscopy (SERS) nanotags and polymerase chain reaction (PCR). SERS provided the sensitivity and spectral multiplexing capability while PCR provided the specificity required for the assay. The method was tested by simultaneous detection of two MRSA specific genes (mecA and femA) amplified from genomic DNA isolated from clinical specimens. Magnetic isolation and rapid duplex detection were used to obtain detectable signal down to 104 input copies within 80 min. This demonstrated the potential of the SERS-PCR based approach for accurate identification of MRSA at an early-diagnosis stage. This study also provides an alternative approach over existing methods for detecting clinical targets without compromising sensitivity and selectivity, and with minimal handling steps. We thus believe this approach will find a broad application in clinic application.