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A method for recording the two phases of dopamine release in mammalian brain striatum slices

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Abstract

Striatal dopamine (DA) release plays an essential role in many physiological functions including motor and non-motor behaviors (such as reward, motivation, and cognition). We have previously reported that, following a single electrical field stimulation, the amperometric recording of DA release from presynaptic terminals in striatal slices (both ventral and dorsal) contains two temporally separated phases. The first phase (direct DA transmission, direct DT) arises from DA terminal release following autologous action potentials (APs), while the second phase (cholinergic transmission-induced DA transmission, CTDT) arises from delayed DA release triggered by the activation of cholinergic interneurons to DA terminals (axon–axon transmission). The millisecond time-resolution of amperometry permits separation of an ∼7 ms latency difference from the single synapse (axon–axon) within the two-phase DA-release (2pDA) signal, and thus the 2pDA signal provides a novel method to study either direct DT, or CTDT, or both. Here, we describe the 2pDA method, including signal recording, processing, analysis, and troubleshooting (anti-artifact). Compared with other DA assays using different stimuli, recording methods, and preparations (such as high performance liquid chromatography or fast scan cyclic voltammetry), 2pDA recording is a novel and powerful physiological recording method for the study of DA transmissions in situ.

Graphical abstract: A method for recording the two phases of dopamine release in mammalian brain striatum slices

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Publication details

The article was received on 30 Sep 2019, accepted on 26 Nov 2019 and first published on 28 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN01941C
Analyst, 2020, Advance Article

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    A method for recording the two phases of dopamine release in mammalian brain striatum slices

    R. Jiao, W. Liu, L. Yin, Z. Qiao, J. Li, L. Zhou, M. Younus, L. Wang, H. Xu and Z. Zhou, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN01941C

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