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A new long-wavelength fluorescent probe for tracking peroxynitrite in live cells and inflammatory sites of zebrafish

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Abstract

Peroxynitrite (OONO), as a reactive oxygen species (ROS), would be mostly profoundly implicated in diseases such as inflammation in organisms. However, bioimaging of ONOO still faces difficulties owing to the shortage of bioimaging and real-time dynamic tracking distribution of ROS in inflammation. To address this challenge, we designed and synthesized a long-wavelength fluorescent probe based on tricyanofuran (ACDM-BE), which exhibits a fast response (response time is 40 s), high selectivity and great sensitivity (LOD is approximately 21 nM) towards ONOO. ACDM-BE was shown to be capable of detecting ONOO in living cells and monitor the changes in ONOO levels under the stimulus of various concentrations of SIN-1 (from 100 to 700 μM), which was successfully tracked by the fluorescence changes in live cells. It is worth noting that ACDM-BE further demonstrated its ability to track the dynamic changes of the level of ONOO in the inflammatory sites of larval zebrafish. Thus, ACDM-BE could be employed as an efficient tool for exploiting the role of ONOO in inflammation in living biosystems.

Graphical abstract: A new long-wavelength fluorescent probe for tracking peroxynitrite in live cells and inflammatory sites of zebrafish

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Supplementary files

Article information


Submitted
27 Sep 2019
Accepted
13 Nov 2019
First published
21 Nov 2019

Analyst, 2020, Advance Article
Article type
Paper

A new long-wavelength fluorescent probe for tracking peroxynitrite in live cells and inflammatory sites of zebrafish

G. Wang, Y. Wang, C. Wang, C. Huang and N. Jia, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN01934K

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