Bio-inspired construction of a semi-artificial enzyme complex for detecting histone acetyltransferases activity

Abstract

Herein, an electrochemical method to detect histone acetyltransferases activity (HAT) has been developed based on the reduction of G-Quadruplex-Cu(II) metalloenzyme activity. A G-quadruplex-Cu(II) metalloenzyme has excellent peroxidase property, generating strong electrochemical signal. In the presence of HAT, it can catalyze substrate peptide acetylation and produce large amounts of Coenzyme A (CoA). The electrochemical signal of G-Quadruplex-Cu(II) is weak due to the competitive combination between G-Quadruplex and CoA with Cu(II), resulting in the direct quantitative detection of HAT. The detection limit for HAT is about 0.14 nM using this strategy and the cost is quite low since the developed assay method is label-free and antibody-free due to the use of low-cost DNA and Cu²⁺. Since this assay method can be employed to detect HAT in serum, it may be useful in disease diagnosis in the future.