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Issue 3, 2020
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Progress in the isolation of aptamers to light-up the dyes and the applications

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Abstract

Light-up small molecule fluorescent dyes play an important role in modern analytical techniques, and they can be widely applied in biochemistry and biomedicine. Aptamers, including single-stranded ribonucleic acids and deoxyribonucleic acids, are molecules that have high affinity for, and can selectively bind to, a specific target, including ions, small molecules, cells, etc. Aptamers are selected in vitro using a process termed SELEX. Light-up dyes have excellent fluorogenic behavior because, although their baseline fluorescence is very low, their fluorescence quantum yield dramatically increases when they bind with a specific aptamer. Aptamer-dye pairs are used in biological imaging and chemical analysis because of their numerous advantages, including their low cost, small size, ease of modification, stability, and low immunogenicity. This paper reviews the progress that has been made in the selection of aptamers for use with light-up dyes and the applications of aptamer-based light-up dyes.

Graphical abstract: Progress in the isolation of aptamers to light-up the dyes and the applications

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Article information


Submitted
16 Sep 2019
Accepted
13 Nov 2019
First published
28 Nov 2019

Analyst, 2020,145, 701-718
Article type
Critical Review

Progress in the isolation of aptamers to light-up the dyes and the applications

T. Gao, Y. Luo, W. Li, Y. Cao and R. Pei, Analyst, 2020, 145, 701
DOI: 10.1039/C9AN01825E

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