Novel immobilization fluorescence capillary analysis method and its applications
The fluorescence capillary analysis (FCA) realized trace-level analysis for micro-volume sample, and it is easy to operate and extremely low in analytical cost and can significantly lessen environmental pollution from wastes of analytical chemistry. FCA has character of green analytical chemistry and has been applied to clinical, biochemical, pharmaceutical, food safety and other fields. FCA basically consists of a micro-volume glass capillary, a capillary holder and an ordinary fluorescence detector. The capillary is not only a container for chemical reaction and detection, but also a carrier to immobilize enzymes, gene probes or reagents, and can be used repeatedly or disposable. In analyzing, the capillary which is modified by functional reagents sucks in a measured liquid to react, and then is inserted into the holder within the fluorescent detector to measure. An immobilized FCA method has been successfully used in determinations of reduced coenzyme I, ethanol in liqueur, lactic acid in dairy products, pyruvic acid and glucose in serum, trace-level sulfated bile acid in urine, ratio of pyruvic/lactic acid in serum, pyruvic acid in cell, and in DNA end-labeled and dyeing methods. Further, FCA could also progress to the capillary array to complete multipurpose simultaneous determination and combine with mobile phones as fluorescence detectors to become a mobile health analytical technology. FCA will produce considerable social benefits in medicine, pharmacy, fermentation food, environmental protection and other fields. So the relevant contents are presented in this tutorial review.