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An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors

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Abstract

AlGaN/GaN high electron mobility transistor (HEMT) biosensors have attracted attention due to their high sensitivity, stability, and fast response characteristics. Some related studies have been explored but a Debye screening problem exists in physiological solutions hindering the detection of bio-macromolecules. Herein, a novel fast analytical platform for electronic enzyme-linked immunosorbent assay (e-ELISA) is proposed based on AlGaN/GaN HEMT with magnetic beads (MBs); MB-based e-ELISA decouples the modified area from the sensing surface to simplify the assay. Combining the advantages of e-ELISA and MBs, the resulting analytical platform presents a sensing capability beyond the Debye-screening limit and a novel ability to be reused. This platform offers a fast response toward prostate specific antigen (PSA) and the lowest concentration of detection is 1 fg mL−1. Compared with conventional AlGaN/GaN HEMT biosensors, it shows higher sensitivity (3.73 μA dec−1) in a linear range (1 fg mL−1 to 1 pg mL−1), which is within the constraints of emergency care applications. The platform's high sensitivity and fast repeatability endow it with great potential for early and rapid diagnosis.

Graphical abstract: An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors

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Article information


Submitted
13 Sep 2019
Accepted
27 Jan 2020
First published
29 Jan 2020

Analyst, 2020, Advance Article
Article type
Paper

An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors

J. Wang, Z. Gu, X. Liu, L. Zhao, H. Peng and J. Li, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN01809C

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