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LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip

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Abstract

Surface plasmon resonance (SPR) nanowire array chips with a microfluidic system are an effective detection method for a rapid test device. This study investigated a capped gold nanowire array and a microfluidic test platform to provide a fundamental understanding of the kinetic binding of SPR nanowires and the surface gold refractive index. The device sensitivity of the SPR nanowire array was 485 nm RIU−1 and the detection limit was 4.1 × 10−5 RIU. Moreover, a kinetic binding analysis also indicated that a peak shift resulted from a specific hybridization of the target molecule with the immobilized probe on the gold nanostructures. The peak shift (red-shift) value of latent membrane protein 1 (LMP1) DNA was 2.21 nm. The results demonstrated that this new method had high sensitivity to detect amplified DNA products without labeling or complex sample treatment. The SPR nanowire chip can detect the PCR products at lower cycle numbers compared to gel electrophoresis due to probe and DNA specificity. Furthermore, the mechanisms of SPR nanowire array fabrication and the detection of the LMP1 gene were studied. The findings can assist in improving the biosensing of DNA-amplified products and in developing rapid detection devices with a small-footprint nanostructured SPR chip.

Graphical abstract: LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip

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Publication details

The article was received on 27 Jul 2019, accepted on 03 Nov 2019 and first published on 25 Nov 2019


Article type: Paper
DOI: 10.1039/C9AN01419E
Analyst, 2020, Advance Article

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    LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip

    C. Chuang, C. Wu, P. Juan, N. Hou, Y. Fan, P. Wei and H. Sheen, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN01419E

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