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Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

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Abstract

Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.

Graphical abstract: Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

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Supplementary files

Article information


Submitted
04 Jun 2019
Accepted
14 Dec 2019
First published
19 Dec 2019

This article is Open Access

Analyst, 2020, Advance Article
Article type
Paper

Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

R. Gaifulina, D. J. Caruana, D. Oukrif, N. J. Guppy, S. Culley, R. Brown, I. Bell, M. Rodriguez-Justo, K. Lau and G. M. H. Thomas, Analyst, 2020, Advance Article , DOI: 10.1039/C9AN01030K

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