Issue 23, 2020

Biointerface topography mediates the interplay between endothelial cells and monocytes

Abstract

Endothelial cell (EC) monolayers located in the inner lining of blood vessels serve as a semipermeable barrier between circulating blood and surrounding tissues. The structure and function of the EC monolayer affect the recruitment and adhesion of monocytes, which plays a pivotal role in the development of inflammation and atherosclerosis. Here we investigate the effect of material wrinkled topographies on the responses of human umbilical vein endothelial cells (HUVECs) and adhesion of monocytes to HUVECs. It is found that HUVEC responses are non-linearly mediated by surface topographies with different dimensions. Specifically, more cell elongation and better cell orientation on the wrinkled surface with a 3.5 μm amplitude and 10 μm wavelength (W10) are observed compared to other surfaces. The proliferation rate of HUVECs on the W10 surface is higher than that on other surfaces due to more 5-ethynyl-2′-deoxyuridine (EdU) detected on the W10 surface. Also, greater expression of inflammatory cytokines from HUVECs and adhesion of monocytes to HUVECs on the W10 surface is shown than other surfaces due to greater expression of p-AKT and ICAM, respectively. This study offers a new in vitro system to understand the interplay between HUVEC monolayers and monocytes mediated by aligned topographies, which may be useful for vascular repair and disease modeling for drug testing.

Graphical abstract: Biointerface topography mediates the interplay between endothelial cells and monocytes

Supplementary files

Article information

Article type
Paper
Submitted
23 Jan 2020
Accepted
28 Mar 2020
First published
06 Apr 2020
This article is Open Access
Creative Commons BY license

RSC Adv., 2020,10, 13848-13854

Biointerface topography mediates the interplay between endothelial cells and monocytes

Y. Liu, W. Deng, L. Yang, X. Fu, Z. Wang, P. van Rijn, Q. Zhou and T. Yu, RSC Adv., 2020, 10, 13848 DOI: 10.1039/D0RA00704H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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