Issue 33, 2020, Issue in Progress

Natural heterogeneous catalysis with immobilised oxidase biocatalysts


The generation of immobilised oxidase biocatalysts allowing multifunctional oxidation of valuable chemicals using molecular oxygen is described. Engineered galactose oxidase (GOase) variants M1 and M3–5, an engineered choline oxidase (AcCO6) and monoamine oxidase (MAO-N D9) displayed long-term stability and reusability over several weeks when covalently attached on a solid support, outperforming their free counterparts in terms of stability (more than 20 fold), resistance to heat at 60 °C, and tolerance to neat organic solvents such as hexane and toluene. These robust heterogenous oxidation catalysts can be recovered after each reaction and be reused multiple times for the oxidation of different substrates.

Graphical abstract: Natural heterogeneous catalysis with immobilised oxidase biocatalysts

Supplementary files

Article information

Article type
07 Apr 2020
18 May 2020
First published
21 May 2020
This article is Open Access
Creative Commons BY license

RSC Adv., 2020,10, 19501-19505

Natural heterogeneous catalysis with immobilised oxidase biocatalysts

A. P. Mattey, J. J. Sangster, J. I. Ramsden, C. Baldwin, W. R. Birmingham, R. S. Heath, A. Angelastro, N. J. Turner, S. C. Cosgrove and S. L. Flitsch, RSC Adv., 2020, 10, 19501 DOI: 10.1039/D0RA03618H

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