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Issue 39, 2020
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Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures

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Abstract

Non-DNA labels are key components for the construction of functional DNA nanostructures. Here, we present a method to graft covalent labels onto DNA origami nanostructures in an enzymatic one-pot reaction. The DNA methyltransferase M.TaqI labels the DNA nanostructures with azide groups, which serve as universal attachment points via click chemistry. Direct labeling with fluorescent dyes is also demonstrated. The procedure yields structures with high fluorescence intensities and narrow intensity distributions. In combination with UV crosslinking it enables the creation of temperature-stable, intense fluorescent beacons.

Graphical abstract: Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures

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Supplementary files

Article information


Submitted
12 May 2020
Accepted
23 Jul 2020
First published
01 Oct 2020

This article is Open Access

Nanoscale, 2020,12, 20287-20291
Article type
Paper

Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures

C. Heck, D. Torchinsky, G. Nifker, F. Gularek, Y. Michaeli, E. Weinhold and Y. Ebenstein, Nanoscale, 2020, 12, 20287
DOI: 10.1039/D0NR03694C

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