Issue 47, 2020

Aqueous core and hollow silica nanocapsules for confined enzyme modules


The development of enzyme modules by coupling several enzymes in confinement is of paramount importance to artificial biological reaction systems for efficient enzymatic reactions. Silica nanocapsules are ideal candidates for loading enzymes. Aqueous core silica nanocapsules have relatively been rarely reported due to the crux of difficulty in forming dense silica shells by interfacial sol–gel reactions. Herein we suggest a one-step synthesis of hollow silica nanocapsules with an aqueous core containing enzymes via a template-free and interfacial condensation method for developing enzyme modules with coupled enzymatic reactions. As a proof-of-concept, we developed enzyme modules for three useful purposes by encapsulating a couple of enzymes: (i) development of a miniature glucose sensor, (ii) protection of living cells, and (iii) regeneration of nicotinamide adenine dinucleotides (NADs). By the modulation of enzymes using silica nanocapsules, more efficient coupled reactions, separation of enzymatic reactions from surroundings, and easy handling of several enzymes by using a single module could be achieved. Therefore, our silica nanocapsules for enzyme modules can be promoted as general platforms for developing artificial nanoreactors.

Graphical abstract: Aqueous core and hollow silica nanocapsules for confined enzyme modules

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Article information

Article type
06 Oct 2020
15 Nov 2020
First published
16 Nov 2020
This article is Open Access
Creative Commons BY license

Nanoscale, 2020,12, 24266-24272

Aqueous core and hollow silica nanocapsules for confined enzyme modules

S. Jo, S. Jiang, R. Graf, F. R. Wurm and K. Landfester, Nanoscale, 2020, 12, 24266 DOI: 10.1039/D0NR07148J

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