Issue 34, 2020

Digital counting of nucleic acid targets using solid-state nanopores

Abstract

Assays targeting biomarkers for the early diagnosis of disease demand a sensing platform with a high degree of specificity and sensitivity. In this work, we developed and characterized a solid-state nanopore-based sensing assay for the detection of short nucleic acid targets with readily customizable nanostructured DNA probe sets. We explored the electrical signatures of three DNA nanostructures to determine their performance as probe sets in a digital counting scheme to quantify the concentration of targets. With these probes, we demonstrate the specific, simultaneous detection of two different DNA targets in a 2-plex assay, and separately that of microRNA-155, a biomarker linked to various human cancers. In addition to specific target detection, our scheme demonstrated the ability to quantify at least six different microRNA concentrations. These results highlight the potential for solid-state nanopores as single-molecule counters for future digital diagnostic technologies.

Graphical abstract: Digital counting of nucleic acid targets using solid-state nanopores

Supplementary files

Article information

Article type
Paper
Submitted
19 May 2020
Accepted
15 Aug 2020
First published
24 Aug 2020

Nanoscale, 2020,12, 17833-17840

Digital counting of nucleic acid targets using solid-state nanopores

E. Beamish, V. Tabard-Cossa and M. Godin, Nanoscale, 2020, 12, 17833 DOI: 10.1039/D0NR03878D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements