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Issue 11, 2020
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Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

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Abstract

Directed guidance of neurites is a pre-requisite for tailor-made designs of interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, and ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques for a cost- and time-effective fabrication of modulated surfaces. In this work, neurite outgrowth of murine cerebellar neurons on 2.5D pathways produced with these methods is studied. Structures of micron-sized steps and grooves serve as cell culture platforms. The effects of contact guidance through topography and chemical guidance through selective poly-D-lysine coating on these platforms are analyzed. As a consequence, the herein presented fabrication approach can be utilized to cultivate and to study low-density neuronal networks in 2.5D configuration with a high degree of order.

Graphical abstract: Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

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Supplementary files

Article information


Submitted
23 Mar 2020
Accepted
14 Jul 2020
First published
14 Jul 2020

This article is Open Access

Nanoscale Adv., 2020,2, 5192-5200
Article type
Paper

Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

C. Fendler, J. Harberts, L. Rafeldt, G. Loers, R. Zierold and R. H. Blick, Nanoscale Adv., 2020, 2, 5192
DOI: 10.1039/D0NA00549E

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