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Issue 23, 2020
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Digital dipstick: miniaturized bacteria detection and digital quantification for the point-of-care

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Abstract

Established digital bioassay formats, digital PCR and digital ELISA, show extreme limits of detection, absolute quantification and high multiplexing capabilities. However, they often require complex instrumentation, and extensive off-chip sample preparation. In this study, we present a dipstick-format digital biosensor (digital dipstick) that detects bacteria directly from the sample liquid with a minimal number of steps: dip, culture, and count. We demonstrate the quantitative detection of Escherichia coli (E. coli) in urine in the clinically relevant range of 102–105 CFU ml−1 for urinary tract infections. Our format shows 89% sensitivity to detect E. coli in clinical urine samples (n = 28) when it is compared to plate culturing (gold standard). The significance and uniqueness of this diagnostic test format is that it allows a non-trained operator to detect urinary tract infections in the clinically relevant range in the home setting.

Graphical abstract: Digital dipstick: miniaturized bacteria detection and digital quantification for the point-of-care

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Supplementary files

Article information


Submitted
06 Aug 2020
Accepted
04 Nov 2020
First published
10 Nov 2020

This article is Open Access

Lab Chip, 2020,20, 4349-4356
Article type
Communication

Digital dipstick: miniaturized bacteria detection and digital quantification for the point-of-care

E. Iseri, M. Biggel, H. Goossens, P. Moons and W. van der Wijngaart, Lab Chip, 2020, 20, 4349
DOI: 10.1039/D0LC00793E

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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