Jump to main content
Jump to site search

Issue 1, 2020
Previous Article Next Article

Tailorable synthesis of heterogeneous enzyme–copper nanobiohybrids and their application in the selective oxidation of benzene to phenol

Author affiliations

Abstract

A protein-direct synthetic route to make nanobiohybrids containing copper nanoparticles (CuNPs) using an enzyme in combination with copper sulfate in aqueous media at room temperature is described. Enzyme–Cu(II) intermediates, rapidly generated in aqueous media, are transformed, induced by the enzyme structure, to facilitate the formation of a particular Cu species, tailoring the size of the nanoparticles formed depending on the experimental conditions, e.g. the pH, reducing step, amount of enzyme or incubation time. This controlled synthesis allows the preparation of tailor-made nanobiohybrids of Cu(0)NPs, Cu(0)/Cu2ONPs, Cu2ONPs or Cu3(PO4)2NPs on the multimilligram scale. The catalytic performance of the novel CuNPs nanobiohybrids was evaluated in relation to the selective C–H functionalization of benzene via direct monohydroxylation using hydrogen peroxide as a green oxidant under mild conditions, in aqueous media and at moderate temperature. Optimization of the amount of hydrogen peroxide, the co-solvent, and the temperature was performed, resulting in the production of 17 mM phenol with 83% selectivity using the Cu-CALB-PHOS-NR nanobiohybrid as a catalyst.

Graphical abstract: Tailorable synthesis of heterogeneous enzyme–copper nanobiohybrids and their application in the selective oxidation of benzene to phenol

Back to tab navigation

Supplementary files

Article information


Submitted
17 Oct 2019
Accepted
26 Nov 2019
First published
27 Nov 2019

This article is Open Access

Catal. Sci. Technol., 2020,10, 196-206
Article type
Paper

Tailorable synthesis of heterogeneous enzyme–copper nanobiohybrids and their application in the selective oxidation of benzene to phenol

N. Losada-García, A. Rodríguez-Otero and J. M. Palomo, Catal. Sci. Technol., 2020, 10, 196
DOI: 10.1039/C9CY02091H

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements