17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers

Lucy J. Rowlands; Adam Marks; John M. Sanderson; Robert V. Law

doi:10.1039/D0CC05466F

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¹⁷O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers†

Lucy J. Rowlands,

^ab Adam Marks,

^b John M. Sanderson

^c and Robert V. Law

*^ab

Author affiliations

* Corresponding authors

^a Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, W12 0BZ London, UK
E-mail: r.law@imperial.ac.uk

^b Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, W12 0BZ London, UK

^c Chemistry Department, Durham University, Durham, UK

Abstract

Cholesterol is a crucial component of biological membranes and can interact with other membrane components through hydrogen bonding. NMR spectroscopy has been used previously to investigate this bonding, however this study represents the first ¹⁷O NMR spectroscopy study of isotopically enriched cholesterol. We demonstrate the ¹⁷O chemical shift is dependent on hydrogen bonding, providing a novel method for the study of cholesterol in bilayers.

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Article information

DOI: https://doi.org/10.1039/D0CC05466F
Article type: Communication
Submitted: 11 Aug 2020
Accepted: 28 Oct 2020
First published: 05 Nov 2020
This article is Open Access

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Chem. Commun., 2020,56, 14499-14502

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¹⁷O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers

L. J. Rowlands, A. Marks, J. M. Sanderson and R. V. Law, Chem. Commun., 2020, 56, 14499 DOI: 10.1039/D0CC05466F

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