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Issue 78, 2020
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A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

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Abstract

The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine 1H–31P NMR.

Graphical abstract: A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

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Supplementary files

Article information


Submitted
20 May 2020
Accepted
25 Aug 2020
First published
25 Aug 2020

This article is Open Access

Chem. Commun., 2020,56, 11665-11668
Article type
Communication

A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

E. Medina-Carmona, L. Varela, A. C. Hendry, G. S. Thompson, L. J. White, J. E. Boles, J. R. Hiscock and J. L. Ortega-Roldan, Chem. Commun., 2020, 56, 11665
DOI: 10.1039/D0CC03612A

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