Issue 78, 2020
From the journal:

Chemical Communications

A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR†

Encarnacion Medina-Carmona,ab   Lorena Varela,a   Alex C. Hendry,a   Gary S. Thompson, ORCID logo a   Lisa J. White,c   Jessica E. Boles,c   Jennifer R. Hiscock ORCID logo c  and  Jose L. Ortega-Roldan ORCID logo *a  

* Corresponding authors

a School of Biosciences, University of Kent, Canterbury, UK
E-mail: J.L.Ortega-Roldan@kent.ac.uk
Tel: +44 (0)1227 824730

b Departamento de Quimica-Fisica, Facultad de Ciencias, Universidad de Granada, Granada 18010, Spain

c School of Physical Sciences, University of Kent, Canterbury, UK

Abstract

The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine 1H–31P NMR.

Graphical abstract: A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

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Article information

DOI
https://doi.org/10.1039/D0CC03612A
Article type
Communication
Submitted
20 May 2020
Accepted
25 Aug 2020
First published
25 Aug 2020
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2020,56, 11665-11668

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A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

E. Medina-Carmona, L. Varela, A. C. Hendry, G. S. Thompson, L. J. White, J. E. Boles, J. R. Hiscock and J. L. Ortega-Roldan, Chem. Commun., 2020, 56, 11665 DOI: 10.1039/D0CC03612A

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