Issue 26, 2020
From the journal:

Chemical Communications

How many cells are enough for single-cell infrared spectroscopy?†

Yadi Wang, ORCID logo ‡abc   Wentao Dai,‡de   Yue Wang, ORCID logo f   Jixiang Liu,e   Zhixiao Liu,a   Yuanyuan Li,de   Xueling Li, ORCID logo *g   Jun Hu ORCID logo ab  and  Junhong Lü ORCID logo *abf  

* Corresponding authors

a Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Science, Shanghai 201800, China
E-mail: lujunhong@zjlab.org.cn

b Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Science, Shanghai 201203, China

c University of Chinese Academy of Sciences, Beijing 100049, China

d Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

e Shanghai Center for Bioinformation Technology & Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai Industrial Technology Institute, Shanghai 201203, China

f College of Pharmacy, Binzhou Medical University, Yantai 264003, China

g Shanghai University of Medicine and Health Sciences, National Engineering Research Center for Nanotechnology, Shanghai 201318, China
E-mail: xlli@nercn.com.cn

Abstract

The influence of cell number on spectral variability among two different cell types and two states was quantitatively analyzed and evaluated by calculating the similarity distance of the single-cell spectral data obtained from synchrotron infrared microspectroscopy. We found that at least 15 cells are required to achieve robust results with 95% confidence.

Graphical abstract: How many cells are enough for single-cell infrared spectroscopy?

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Article information

DOI
https://doi.org/10.1039/D0CC00067A
Article type
Communication
Submitted
04 Jan 2020
Accepted
25 Feb 2020
First published
26 Feb 2020

Chem. Commun., 2020,56, 3773-3776

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How many cells are enough for single-cell infrared spectroscopy?

Y. Wang, W. Dai, Y. Wang, J. Liu, Z. Liu, Y. Li, X. Li, J. Hu and J. Lü, Chem. Commun., 2020, 56, 3773 DOI: 10.1039/D0CC00067A

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