Issue 25, 2020

Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases

Abstract

Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting ‘interactome’. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by ‘privileged’ synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for ‘interactome’ profiling.

Graphical abstract: Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases

Supplementary files

Article information

Article type
Communication
Submitted
20 Dec 2019
Accepted
19 Feb 2020
First published
19 Feb 2020

Chem. Commun., 2020,56, 3641-3644

Author version available

Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases

S. Wagner, B. Sudhamalla, P. Mannes, S. Sappa, S. Kavoosi, D. Dey, S. Wang and K. Islam, Chem. Commun., 2020, 56, 3641 DOI: 10.1039/C9CC09891G

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