Jump to main content
Jump to site search


Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity

Author affiliations

Abstract

Celastrol is a natural bioactive compound extracted from the medicinal plant Tripterygium wilfordii Hook F. It exhibits immunosuppressive, anti-inflammatory, and antioxidant activities. Cisplatin is a commonly used chemotherapeutic drug in the treatment of a wide range of tumors. Although very effective therapeutically, it can cause nephrotoxicity leading to dose reduction or discontinuation of treatment. This study aims to clarify the therapeutic potential of celastrol in cisplatin-induced nephrotoxicity. The possible protective effects of celastrol pretreatment against cisplatin-induced oxidative stress and genotoxicity were investigated. A rat kidney epithelial cell line NRK-52E was pretreated with the desired concentrations of celastrol (200 nM, 100 nM, and 50 nM) for 24 h. The cells were treated with 50 μM cisplatin for a further 24 h to see whether cisplatin caused the same or less toxicity compared to the vehicle control group. Alkaline comet assay was performed for genotoxicity assessment. Genotoxicity evaluation revealed that celastrol caused a statistically significant reduction in DNA damage. Oxidative stress parameters were evaluated by measuring the glutathione (GSH) and protein carbonyl (PC) levels and also by measuring the enzyme activities of glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) enzymes. Celastrol pretreatment increased the GSH content of the cells and ameliorated the protein carbonylation level. Likewise, celastrol pretreatment improved the GR and CAT activities. However, no significant difference was observed in GPx and SOD activities. In the light of these findings, celastrol treatment could be a therapeutic option to reduce cisplatin-induced nephrotoxicity. Further studies are needed for the clarification of its therapeutic potential.

Graphical abstract: Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity

Back to tab navigation

Publication details

The article was received on 11 Jun 2019, accepted on 29 Jul 2019 and first published on 31 Jul 2019


Article type: Paper
DOI: 10.1039/C9TX00141G
Toxicol. Res., 2019, Advance Article

  •   Request permissions

    Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity

    T. Boran, A. Gunaydin, A. T. Jannuzzi, E. Ozcagli and B. Alpertunga, Toxicol. Res., 2019, Advance Article , DOI: 10.1039/C9TX00141G

Search articles by author

Spotlight

Advertisements