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Real-Time Monitoring of Intracellular Nitric Oxide Using a Long-Wavelength-Emitting Probe via One-Photon or Two-Photon Excitation

Abstract

In biological imaging, the use of long-wavelength-emitting (> 650 nm) fluorescent probes can significantly reduce the interference from cell auto-fluorescence and thus greatly improve the imaging resolution because the intrinsic biomolecules rarely absorb light and emit fluorescence in the long-wavelength region. In this work, a long-wavelength-emitting two-photon fluorescent probe (TTNO) for nitric oxide (NO) detection in living cells is designed and synthesized. TTNO shows ~28-fold enhanced fluorescence intensity (at 658 nm) with a low detection limit of 9 nM, a fast response to NO within ~40 seconds and a high selectivity for NO. In addition, it is worth mentioning that TTNO can also be excited with near-infrared light via two-photon absorption. Its biological application for real-time imaging of exogenous and endogenous NO in living cells is successfully demonstrated by using one-photon and two-photon microscopy, respectively.

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Publication details

The article was received on 11 Dec 2018, accepted on 12 Feb 2019 and first published on 13 Feb 2019


Article type: Paper
DOI: 10.1039/C8TC06238B
Citation: J. Mater. Chem. C, 2019, Accepted Manuscript

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    Real-Time Monitoring of Intracellular Nitric Oxide Using a Long-Wavelength-Emitting Probe via One-Photon or Two-Photon Excitation

    C. Tang, M. Wang, X. Shang, X. Chen, D. Huang and Q. Zheng, J. Mater. Chem. C, 2019, Accepted Manuscript , DOI: 10.1039/C8TC06238B

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