Biomimetic Bone Tissue Engineering Hydrogel Scaffolds Constructed Using Ordered CNTs and HA Induce the Proliferation and Differentiation of BMSCs
Bone tissue engineering scaffolds have become the promising potential treatment for bone defects as they expedite bone in healing. Carbon-nanotubes-hydroxyapatite (CNTs-HA) composite can accelerate the growth of cells. However, molecular organized arrangement of organic and inorganic components is one of the most important biochemical phenomena in the formation of bone. This study aimed to prepare ordered CNTs-HA scaffolds by applying agarose gel electrophoresis to imitate a biomimetic parallel pattern of collagens and hydroxyapatite hydrogel scaffolds (AG-Col-o-CNTs). Significant improvements were presented in the mechanical properties of the scaffolds and cell growth in vitro or in vivo. The results showed that AG-Col-o-CNTs scaffolds accelerated the proliferation and differentiation of bone mesenchymal stem cell lines. In addition, bone defects were repaired when scaffolds were transplanted after 28 and 56 days in vivo. The superior performance of the ordered AG-Col-o-CNTs scaffolds indicated that they had the enormous potentials for bone tissue engineering.