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Time-resolved near-infrared phosphorescent iridium(III) complex for fast and highly specific peroxynitrite detection and its bioimaging application

Abstract

Peroxynitrite (ONOO−), one of the reactive oxygen/nitrogen species (ROS/RNS) found in vivo, plays crucial roles in many physiological and pathological processes. The ability to selectively and sensitively determine ONOO− in vivo is important for the understanding of its biological roles. Thus, by utilizing the excellent chemical stability and photostability, high luminescence efficiency, and long luminescence lifetime of iridium complex, we developed a novel near-infrared (NIR) phosphorescent iridium(III) complex (FNO2) probe to detect ONOO− within seconds. The probe FNO2 had better selectivity towards ONOO− over other interfering-biomolecules, including O2− and ClO−. Moreover, it possessed a long luminescence lifetime, that was able to successfully eliminate the interference from background fluorescence in vitro (simulated by Rhodamine B) in time-resolved emission spectra. Finally, in addition to its low cytotoxicity, the probe FNO2 showed emission wavelengths in the NIR region and was able to specifically sense ONOO− induced in living cells and inflamed mouse models.

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Publication details

The article was received on 07 Aug 2019, accepted on 28 Oct 2019 and first published on 29 Oct 2019


Article type: Paper
DOI: 10.1039/C9TB01673B
J. Mater. Chem. B, 2019, Accepted Manuscript

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    Time-resolved near-infrared phosphorescent iridium(III) complex for fast and highly specific peroxynitrite detection and its bioimaging application

    Y. Li, Y. Wu, L. Chen, H. Zeng, X. Chen, W. Lun, X. Fan and W. R. Wong, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C9TB01673B

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