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Direct 3D printing of tough hydrogel incorporated with carbon nanotubes for bone regeneration

Abstract

The emerging three-dimensional (3D) printing technique has shown prominent advantages to fabricate hydrogel‐based tissue scaffolds for the regeneration of bone defects. Here, a tough polyion complex (PIC) hydrogel was synthesized, and multiwalled carbon nanotubes (MWCNTs) was incorporated into PIC matrix to form PIC/MWCNT biohybrid hydrogel, which was manufactured into 3D scaffolds by extrusion-based 3D printing for bone defect repair. To the best of our knowledge this is the first study to combine CNTs with PIC hydrogels as the biohybrid scaffolds for bone repair. The results of in vitro cell culture demonstrated that the PIC/MWCNT scaffolds exhibited good biocompatibility to rat bone marrow-derived mesenchymal stem cells (rBMSCs) and facilitates the osteogenic differentiation of rBMSCs. Moreover, rBMSCs cultured on the PIC/MWCNT scaffolds exhibited a higher degree of osteogenic differentiation than those cultured on PIC scaffolds, in terms of mineralized matrix formation and osteogenesis-related gene upregulated. The in vivo experiments in a calvarial defect model of Sprague−Dawley (SD) rats revealed that PIC/MWCNT scaffolds significantly promoted regeneration of calvarial defect healing. These findings suggest that PIC hydrogel is the potential scaffold material for bone regeneration, and the addition of MWCNTs provides further enhancement of bone repair efficiency by PIC/MWCNT scaffolds.

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Publication details

The article was received on 18 Jul 2019, accepted on 10 Oct 2019 and first published on 10 Oct 2019


Article type: Paper
DOI: 10.1039/C9TB01494B
J. Mater. Chem. B, 2019, Accepted Manuscript

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    Direct 3D printing of tough hydrogel incorporated with carbon nanotubes for bone regeneration

    H. Cui, Y. Yu, X. Li, Z. Sun, J. Ruan, Z. L. Wu, J. Qian and J. Yin, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C9TB01494B

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