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The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation

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Abstract

Adsorbents with synchronously high protein adsorption performance and a facile synthetic route are highly desired in protein separation. In this study, a facile yet effective strategy to develop porous chitosan microspheres (PCMs) with high specific surface area (SSA) using agarose as the pore-forming agent is reported. Through heat treatment, the agarose chains in the chitosan/agarose composite microspheres (CAM) were removed, leading to the generation of nanopores/nanochannels and the improvement of SSA. The obtained PCMs showed hierarchical porous structure and a maximum SSA of 246.48 m2 gāˆ’1. For the application of PCMs as a protein adsorbent, by modification, the resultant immobilized Cu2+ affinity adsorbent (denoted as Cu2+PCM-15) exhibited a high adsorption capacity (301.88 mg gāˆ’1), fast adsorption rate (reaching equilibrium in less than 15 min), and excellent adsorption selectivity for BHb. Together with its environmental-friendliness, and abundant biomass chitosan and agarose, the as-prepared affinity adsorbent with high performance has great application potential in the field of bioseparation.

Graphical abstract: The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation

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Publication details

The article was received on 10 Jun 2019, accepted on 06 Aug 2019 and first published on 06 Aug 2019


Article type: Paper
DOI: 10.1039/C9TB01157A
J. Mater. Chem. B, 2019, Advance Article

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    The construction of porous chitosan microspheres with high specific surface area by using agarose as the pore-forming agent and further functionalized application in bioseparation

    L. Qiao, L. Zhao, C. Liang and K. Du, J. Mater. Chem. B, 2019, Advance Article , DOI: 10.1039/C9TB01157A

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