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In situ generated chromophore as the indicator for background-free sensing strategy of hydrazine with high sensitivity with in vitro and in vivo applications

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Abstract

A chromophore is essential to a classic molecular probe, but its intrinsic optical properties can trigger a spectral background, which is considered to produce a negative influence on detection sensitivity. Hence, developing spectral sensing assays with chromophores generated in situ by probes and analytes could realize detection. Herein, we elaborated a novel proposal based on the in situ condensation reaction between 4-diethylaminobenzaldehyde (DEASA) and hydrazine readily promoted by CTAB micelles, resulting in new chromophores with intramolecular hydrogen bonds generated in situ acting as indicators for hydrazine. A background-free detection pattern was, therefore, formulated, along with a relatively low detection limit of 0.42 nM. This avoided tedious preparation and the use of organic solvents during the experiments. In addition, extensive studies, including actual water/serum samples, living cells, and even mouse imaging, revealed that the DEASA probe and the well-designed in situ sensing scheme exhibited good performance regarding future applications in terms of sensitivity, anti-interference capability, and imaging stability.

Graphical abstract: In situ generated chromophore as the indicator for background-free sensing strategy of hydrazine with high sensitivity with in vitro and in vivo applications

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Publication details

The article was received on 21 Apr 2019, accepted on 05 Jul 2019 and first published on 09 Jul 2019


Article type: Paper
DOI: 10.1039/C9TB00769E
J. Mater. Chem. B, 2019, Advance Article

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    In situ generated chromophore as the indicator for background-free sensing strategy of hydrazine with high sensitivity with in vitro and in vivo applications

    P. Zhang, Y. Gong, Q. Zhang, X. Guo and C. Ding, J. Mater. Chem. B, 2019, Advance Article , DOI: 10.1039/C9TB00769E

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